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Energy and Environment Cabinet

Division of Water

Division of Water
Standard Operating Procedures

Standard Operating Procedure (SOP) manuals are important tools for achieving quality assurance practices in the Division of Water (DOW).  SOPs describe specific methods and procedures used in all aspects of environmental planning and monitoring projects. Most SOPs utilized in DOW activities are developed internally. These procedures were written to meet state obligations and requirements for monitoring and analyzing data.

The use of these SOPs by outside organizations and agencies should be coordinated with a DOW scientist to ensure that the correct application of these protocols and templates is performed.

The DOW is in the process of revising most of its SOPs for environmental monitoring programs. The most current SOPs are listed in the table below, with links provided to the documents. SOPs that are not linked are currently in progress and should be available soon.

If a procedure is not listed in the current table, it is possible that it was covered in a prior document and can be located in the historic SOP section beneath the table.

Contact the specific project biologist or Lisa Hicks (the quality assurance officer) for assistance in identifying the most current document to use.

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Current SOPs

The following two tables provide the most current SOPs used in biological, physical, chemical and habitat monitoring and assessment.


  
Title
  
Description
DOWSOP0300107/09/2009
Document, track and control all types of samples
DOWSOP0300203/27/2009
Development procedure for pathogen TMDLs
DOWSOP0300305/01/2011
Sampling and collection of macroinvertebrates in wadeable streams
DOWSOP0300404/02/2009
Planning procedures for TMDL monitoring projects
DOWSOP0300505/07/2009
Processing and identification of macroinvertebrates
DOWSOP03006
Sediment Monitoring
in progress
Monitoring for sediments
DOWSOP0300705/07/2009
Processing and identification of fish
DOWSOP0300801/01/2010
Sampling and collection methods of fish in wadeable streams
DOWSOP0300903/01/2009
Identification and enumeration of diatoms
DOWSOP0301003/01/2009
Processing of diatom samples
DOWSOP0301103/01/2009
Sampling and collection procedures for benthic algae in wadeable streams
DOWSOP0301203/01/2009
Assessment of habitat for benthic algae in wadeable streams
DOWSOP0301401/01/2009
Process of calibration and use of in-situ water quality meters
DOWSOP0301501/10/2011
Sampling surface waters in streams
DOWSOP0301609/19/2013
​Document writing, draft stages, public notice and submittal process for TMDL documents.
DOWSOP03017
Bacteriological Sampling (see DOWSOP03015)
in progress
Sampling for pathogens
DOWSOP0301905/01/2010
Procedure for measuring stream discharge
DOWSOP0302405/11/2011
Evaluating habitat conditions in wadeable waters in support of water quality assessments.​
DOWSOP0302505/01/2011
Laboratory procedures for the analysis of total coliforms and E coli bacteria.​
DOWSOP0303104/09/2014

​​SOP for collecting fish tissue in determining selenium concentrations under a KPDES permit

DOWSOP0901403/02/2011
Procedures for handling and documenting using groundwater Chain-of-Custody
DOWSOP0901503/02/2011
Procedures for safe sampling of groundwater​
unassigned
Sampling Lake and Reservoir Systems
in progress
Sampling lake systems
 

 Index Calculation Documents

 
Document
Description
This document is used to make macroinvertebrate assessments statewide. The four MBI templates listed below are multi-metric indices that use macroinvetebrates as bioindicators for stream health. Each template includes a description of how to use the metric.
This document outlines a multi-metric index that uses fish as the bioindicator for assessing stream health.  The KIBI provides a numerical value and corresponding narrative classification for headwater and wadeable streams.  A  KIBI template has been developed to simplify the calculation process.  An  overview of the KIBI document and template is also available.

Historical SOPs

If a procedure or method is not found in the current section of SOPs, then the use of the historical SOPs listed below may be necessary. Check with your project scientist for use of appropriate documents.

Document
 2002 Methods for Assessing Biological Integrity of Surface Waters in Kentucky -- This SOP is a version from 2002 for all biological monitoring and should only be used in reference for past projects.
2008 Methods for Assessing Biological Integrity of Surface Waters in Kentucky -- This SOP is a version from 2008 of all biological monitoring methods. This file includes the  main document and the  appendices.
 A Macroinvertebrate Bioassessment Index (MBI) for Headwater Streams of the Eastern Coalfield Region, Kentucky -- This document delineates the methods DOW uses to assess streams for macroinvertebrates in the eastern Kentucky coalfield region.
 Kentucky Ambient/Watershed Water Quality Monitoring Standard Operating Procedure Manual -- This manual describes how water samples are collected, preserved and delivered to a laboratory for analysis, and should generally only be used for reference purposes.